쥐 등 모델에서의 poly-(L)-lactic acid (PLLA) 차단막과 EGF가 탑재된 PLLA 차단막의 흡수도 및 생체 친화성 평가
Evaluation of degradation pattern and biocompatibility of PLLA (poly-(L)-lactic acid) membrane and EGF (epidermal growth factor) loaded PLLA membrane in rat dorsal models
김유경, 안은철, 권미경, 윤지훈, 허영구, 차재국, 이중석, 정의원, 최성호,
소속 상세정보
김유경 ( Kim You-Kyoung ) - 연세대학교 치과대학 치주과학교실
안은철 ( An Yin-Zhe ) - 연세대학교 치과대학 치주과학교실
권미경 ( Kwon Mi-Kyung ) - 네오 바이오텍 R&D 센터
윤지훈 ( Yoon Ji-Hoon ) - 네오 바이오텍 R&D 센터
허영구 ( Heo Young-Ku ) - 네오 바이오텍 R&D 센터
차재국 ( Cha Jae-Kook ) - 연세대학교 치과대학 치주과학교실
이중석 ( Lee Jung-Seok ) - 연세대학교 치과대학 치주과학교실
정의원 ( Jung Ui-Won ) - 연세대학교 치과대학 치주과학교실
최성호 ( Choi Seong-Ho ) - 연세대학교 치과대학 치주과학교실
KMID : 1034220160080010005
Abstract
The aim of this study was to compare the biodegradation including biocompatibility of differently made membranes, PLLA (poly-(L)-lactic acid membrane), EGF (epidermal growth factor) loaded PLLA membrane and Biogide (non-cross linked collagen membrane). 30 Wistar white rats (12 weeks of age, weighing about 350 g) were used in total surgical procedures. Totally, 90 membranes were allocated in representing 3 groups: C: Control (Biogide® (type I and III porcine collagen membrane, Geistlich-Pharm, Wolhusen, Switzerland), P: PLLA (poly-(L)-lactic acid) and E: E-PLLA (EGF-(epidermal growth factor) loaded poly-(L)-lactic acid). These groups were experimentally divided in five periods: 1, 2, 4, 8 and 12 weeks. The membranes were randomly allocated in subcutaneous pouches separately on the back of the 30 rats. After 1, 2, 4, 8 and 12 weeks, the rats were sacrificed and specimen were prepared for histologic and histometric analysis. The following parameters were evaluated : (1) residual membrane thickness, (2) the morphology of membranes, (3) tissue
integration and foreign body reaction. Histologically, the residual membranes were gradually degraded in control group showing tissue integration with surrounding tissue but the almost all membranes of PLLA and E-PLLA group were not degraded. The thickness of membranes decreased continuously in control group, however thickness of PLLA and E-PLLA increased due to amorphous area from its fragmented polymer. Connective tissue integration was achieved in control group however complete separation was observed in PLLA and E-PLLA group. No vascularization was found in both experimental group. One inflammatory reaction was observed in 8 weeks of PLLA group. Within the limits of the present study, degradation of PLLA membranes was much slower than control membranes. Space maintenance and cell occluding ability of PLLA is so superior that PLLA membrane can be used in cases which require high space-maintaining capacity in GTR and GBR. However biocompatibility of PLLA was not completely proved, therefore enhancement of biocompatibility is needed. In this study, E-PLLA enhanced wound healing and tissue regeneration. Long-term evaluation and studies to prove degradation ratio of PLLA in further study.
키워드
Biocompatibility; Collagen membrane; Epidermal growth factor; Guided tissue regeneration; Poly-(L)-lactic acid
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